circrna expression microarray slide arraystar human circrna array v2 [8 × 15 k]  (Arraystar inc)

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Blocking Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing